Lifeboat Foundation

(Medical Xpress)—A large team of researchers from a host of research facilities across Japan has found some genetic variants in some cancer cells that lead to enhanced PD-L1 protein production—which results in increased protection against attacks by the immune system. In their paper published in the journal Nature, the team describes their sequencing study involving adult T-cell leukemia/lymphoma cases, what they found and the possibility that such variants could be used as identifying markers in cancer patients.

Prior studies have shown that an increase in the expression of the protein PD-L1 by cancer cells confers enhanced protection against attacks by the human immune system—PD-1 receptors on T cells bind with PD-L1 causing the immune cells to become unresponsive, preventing them from attacking tumors. In this new effort, the researchers conducted a genetic analysis of a particular type of cancer cell to learn more about the genetic process involved in causing an increase in expression of PD-L1.

The team conducted whole-genome sequencing on samples given by 49 adult patients suffering from leukemia or lymphoma, looking specifically for variations that might account for an increase in expression of PD-L1. In so doing, they found that variations such as duplications, inversions or translocations in 13 of the samples, representing 27 percent of those tested, existed on a certain part of chromosome 9, which prior research had found was the part of the genome responsible for the expression of PD-L1. They report that such alterations seemed to cut off the gene’s 3’ untranslated region of the protein and in some cases led to rearranging the gene’s open reading frame, which allowed more of the protein to be expressed.

Continue reading “Genetic variants isolated that lead to enhanced PD-L1 protein production in cancer cells” »

“The possibility to selectively activate genes using various engineered variants of the CRISPR-Cas9 system left many researchers questioning which of the available synthetic activating Cas9 proteins to use for their purposes. The main challenge was that all had been uniquely designed and tested in different settings; there was no side-by-side comparison of their relative potentials,” said George Church, Ph.D., who is Core Faculty Member at the Wyss Institute for Biologically Inspired Engineering at Harvard University, leader of its Synthetic Biology Platform, and Professor of Genetics at Harvard Medical School. “We wanted to provide that side-by-side comparison to the biomedical research community.”

In a study published on 23 May in Nature Methods, the Wyss Institute team reports how it rigorously compared and ranked the most commonly used artificial Cas9 activators in different cell types from organisms including humans, mice and flies. The findings provide a valuable guide to researchers, allowing them to streamline their endeavors.

The team also included Wyss Core Faculty Member James Collins, Ph.D., who also is the Termeer Professor of Medical Engineering & Science and Professor of Biological Engineering at the Massachusetts Institute of Technology (MIT)’s Department of Biological Engineering and Norbert Perrimon, Ph.D., a Professor of Genetics at Harvard Medical School.

Continue reading “A Guide to CRISPR Gene Activation” »

Oct4, a gene, thought to be inactive in adults, may actually play a vital role in preventing heart attacks and strokes and could also delay some of the effects of ageing, scientists have found. They said the gene could also prove critical in the field of regenerative medicine.

Representative photo.

AR for plastic surgery.

ILLUSIO, the next generation in computer imaging for plastic surgery, will be presenting at the 2016 Virtual Reality Summit in Seoul, South Korea on June 22. The conference is expected to attract thousands of people interested in the latest applications for virtual reality and augmented reality.

ILLUSIO CEO Ethan Winner will present the Company’s use of augmented reality for plastic surgery imaging. ILLUSIO combines the latest in 3D augmented reality technology with real-time morphing animation, providing a platform for plastic surgeons and their patients to visually communicate.

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More information on ANTs.

In the minuscule world of nanotechnology, big steps are rare. But a recent development has the potential to massively improve our lives: an engine measuring 200 billionths of a metre, which could power tiny robots to fight diseases in living cells.

Life itself is proof of the extreme effectiveness of nanotechnology — the manipulation of matter on a molecular or atomic scale — in which DNA, proteins and enzymes can all be considered as machinery. In fact, researchers have managed to make micro-propellers using tiny strands of DNA. These strands can be stitched together so freely and precisely that the practise is known as “DNA origami”. However, DNA origami lacks force and operational speed (it takes time measurable in seconds), reducing its robotic function.

Continue reading “Powering nanotechnology with the world’s smallest engine” »

US biochemical engineer Frances Arnold on Tuesday won a million-euro technology prize in Finland for her work on “directed evolution”, a method of rewriting DNA to improve medicines and develop green fuels.

“Frances Arnold receives the 2016 Millennium Technology Prize in recognition of her discoveries that launched the field of ‘directed evolution’, which mimics natural evolution to create new and better proteins in the laboratory,” the Technology Academy Finland, which awards the prize at two-year intervals, said in a statement.

Arnold, 59, who is a professor of chemical engineering at California Institute of Technology, said her work made it possible to “solve human problems”, such as replacing toxic chemicals like fossil fuels.

Cientistas de Harvard University criou uma nova plataforma para superar a resistência aos antibióticos.

For the first time ever, scientists were able to successfully cut out the HIV genes from live animals, and they had over a 50% success rate.

A significant milestone was achieved today in the fight against HIV—scientists led by Kamel Khalili of the Comprehensive NeuroAIDS Center at Temple University just reported that, for the first time, HIV genes have been successfully eliminated from the genomes of animals infected with the virus.

Continue reading “HIV Genes Have Been Cut Out of Live Animals Using CRISPR” »

The physical limitations of existing materials are one of main problems when it comes to flexible electronics, be it wearables, medical or sports tech. If a flexible material breaks, it either stays broken, or if it has some self-healing properties it may continue to work, but not so well. However, a team from Penn State have creating a self-healing, flexible material that could be used inside electronics even after multiple breaks.

The main challenge facing researchers led by Professor Qing Wang, was ensuring that self-healing electronics could restore “a suite of functions”. The example used explains how a component may still retain electrical resistance, but lose the ability to conduct heat, risking overheating in a hypothetical wearable, which is never good. The nano-composite material they came up with was mechanically strong, resistant against electronic surges, thermal conductivity and whilst packing insulating properties. Despite being cut it in half, reconnecting the two parts together and healing at a higher temperature almost completely heals where the cut was made. The thin strip of material could also hold up to 200 grams of weight after recovering.