CRISPR Gene-Editing Shows Promise As HIV Cure, Research Shows : Shots — Health News Researchers safely used CRISPR gene-editing techniques in a patient with HIV. The research provides evidence the approach may be promising for treating HIV infection.
The fruit flies in Noah Whiteman’s lab may be hazardous to your health.
Whiteman and his University of California, Berkeley, colleagues have turned perfectly palatable fruit flies—palatable, at least, to frogs and birds—into potentially poisonous prey that may cause anything that eats them to puke. In large enough quantities, the flies likely would make a human puke, too, much like the emetic effect of ipecac syrup.
That’s because the team genetically engineered the flies, using CRISPR-Cas9 gene editing, to be able to eat milkweed without dying and to sequester its toxins, just as America’s most beloved butterfly, the monarch, does to deter predators.
New interview with author and researcher Dr. Josh Mitteldorf who runs the aging research blog Aging Matters.
Interview with author and researcher Dr. Josh Mitteldorf who runs the aging research blog ‘Aging Matters’.
Dr. Josh Mitteldorf is an evolutionary biologist and a long-time contributor to the growing field of aging science. His work in this field has focused on theories of aging. He asks the basic question: why do we age and die?
Continue reading “Josh Mitteldorf — Cracking the Aging Code” »
A breast cancer drug has been used to double the survival of men with advanced prostate cancer, becoming the first successful precision medicine for the disease.
Doctors at the Royal Marsden Hospital who conducted the trial say the results amount to a “revolution” in prostate cancer care.
They conducted genetic testing on more than 4,400 patients to identify those with one or more of 15 types of DNA fault.
It’s the promise of stem cell medicine: Someday soon, clinics will rebuild diseased or broken hearts, kidneys, pancreases or blood by growing and reprogramming human cells, then adding them back to the bodies of the patients they came from.
If only it were that easy.
After two decades of human stem cell research, researchers have learned how to create what appear to be reasonably functional versions of several types of cells, first using genetic tricks to turn cells back to an uncommitted state and then molding them into the type of cell needed — say, an insulin-producing cell or a particular kind of nerve cell. And many early clinical trials of stem cell medicine have shown genuinely promising results.
The Columbia team behind the revolutionary 3D SCAPE microscope announces today a new version of this high-speed imaging technology. In collaboration with scientists from around the world, they used SCAPE 2.0 to reveal previously unseen details of living creatures—from neurons firing inside a wriggling worm to the 3D dynamics of the beating heart of a fish embryo, with far superior resolution and at speeds up to 30 times faster than their original demonstration.
These improvements to SCAPE, published today in Nature Methods, promise to impact fields as wide ranging as genetics, cardiology and neuroscience.
Why is having faster, 3D imaging so valuable? “The processes that drive living things are dynamic and ever-changing, from the way an animal’s cells communicate with one another, to how a creature moves and changes shape,” said Elizabeth Hillman, Ph.D., a principal investigator at Columbia’s Mortimer B. Zuckerman Mind Brain Behavior Institute and the paper’s senior author. “The faster we can image, the more of these processes we can see—and imaging fast in 3D lets us see the whole biological system, rather than just a single plane, offering a clear advantage over traditional microscopes.”
Some of the most important tools in the toolbox of modern cell biologists are special chunks of DNA that act like spies, reporting on the cell’s function. The markers, known as reporter genes, allow researchers to get a sense for what cells are doing by watching genetic programs embedded in their DNA turn on and off.
Reporter genes work by encoding proteins that can be seen from outside the cell. One particularly popular reporter gene encodes something called the green fluorescent protein (GFP), which, true to its name, is a protein that glows bright green. So, if a researcher wants to learn more about how cells become neurons, they can insert the GFP gene alongside a neuronal gene into an embryo’s DNA. When the embryo’s cells turn on the neuron gene, they will also express the GFP gene, and the cells will glow green, making it easy for the researcher to see that the genetic program that encodes neuron formation is active.
As useful as this technique has been, it has a big limitation: Because light does not penetrate well through most living tissue, the GFP gene cannot be used for monitoring the activity of cells deep inside an organism. But now, Caltech’s Mikhail Shapiro has a solution. A team consisting of Shapiro, professor of chemical engineering and investigator with the Heritage Medical Research Institute, graduate student Arash Farhadi, and their colleagues, has developed a reporter gene that allows them to see genetic activity using ultrasound, which can penetrate deeply through tissue, instead of light.
Creating a transgenic mouse demonstrating the rescue of Mitochondrial DNA mutations in mammals. We will express the mitochondrial ATP8 gene from the nucleus as proof of concept towards gene therapies for mtDNA mutations.
A new study shows that scientists might be able to not only slow the process of aging but actually reverse it, Benjamin Button-style.
Volunteers in a California study were given a cocktail of three common drugs for one year— a growth hormone and two diabetes medications. Scientists had been testing the drugs in the hope of regenerating the thymus gland.
But upon further analysis, they found that participants had lost an average of 2.5 years on their “epigenetic clock,” measured by analyzing marks on a person’s genomes, according to the journal Nature. Participants’ immune systems also showed signs of rejuvenation.
